DIRM - DiponEd Institute of Regenerative Medicine (DIRM) | PhD | CRM | FRCM

DIRM Syllabus And Practical’s

Course	Topics
Cell Biology	Cell and Organelle Structure and Function Cell Cycle Cell Signaling Cell Metabolism Cellular Aging
Developmental Biology	Mechanism of Development Cell Type Determination, Organogenesis, and Morphogenesis The Blastocytes and Embryonic Stem Cells Alternative Sources of Pluripotent Stem Cells Mechanisms of Regeneration
Physiology and Anatomy	Organization of Human Body and Cell Types Skeletal System, Skeleton, and Joints The Muscle and Muscular System The Central Nervous System and the Peripheral Nervous System The Endocrine System
Immunology	Anatomy of Immune System Acute and Chronic Inflammation Dendritic Cells and Vaccine Host Defense Against Infections Angiogenesis and Immune Regulation
Cancer Biology and Infectious Diseases	Origin and Overview of Cancer Cancer Immunology Cancer Systems Biology Cancer Diagnostics and Treatments Infectious Agents and Cancer
Molecular Biology	DNA Replication, Recombination, and Repair Fundamentals of Gene Structure and Eukaryotic Transcription Effect of Chromatin on Gene Regulation Analysis of Transcriptional Networks by Proteomics and Functional Genomics Mechanisms and Functions of RNA Interference (RNAi) and Micro RNA

Course	Topics
Basics of Stem Cell Biology	Introduction to the Concepts of Stem Cell Biology Mesenchymal and Hematopoietic Stem Cells Neural, Cardiac, Breast, Epithelial, and Stem Cells Stem Cells and Cancer Stem Cells and Regenerative Medicine Stem Cell Technologies, Commercialization, and Treatment
Stem Cell Research	Introduction to working with hematopoietic progenitor cells, neural stem cells, mammary stem cells, mesenchymal stem cells, and human embryonic and induced pluripotent stem cells Ex-vivo Expansion of Mesenchymal Stem Cells Stem Cells Reprogramming Stem Cell Banking Stem Cell and Regenerative Medicine
Basics of Transplantations and Blood Transfusions	Basic Immunology of Transplantation Principles of immunosuppressive therapy; GVHD Bioethics in transplantation: ethical, social, legal, and religious aspects The Role of Blood Service and Alternate Blood Transfusions Blood Transfusion Management
Hematology/Bone Marrow Transplantations	Overview of Autologous and Allogenic Stem Cell Transplantations Bone Marrow Development and Function Ethical Issues in Bone Marrow Transplantations Immunological Approaches to Treating Bone Marrow Cancers Type of Transplants and Donor Choices
Trauma Injuries	Overview on Trauma Injuries Platelet-Rich Plasma (PRP): From Basic Science to Clinical Applications Sports Trauma Management Head Trauma Treatment and Management Traumatic Injuries: Diagnosis, Treatment, and Complications

Course	Topics
Basics of Stem Cell Biology	Introduction to the Concepts of Stem Cell Biology Mesenchymal and Hematopoietic Stem Cells Neural, Cardiac, Breast, Epithelial, and Stem Cells Stem Cells and Cancer Stem Cells and Regenerative Medicine Stem Cell Technologies, Commercialization, and Treatment
Stem Cell Research	Introduction to working with hematopoietic progenitor cells, neural stem cells, mammary stem cells, mesenchymal stem cells, and human embryonic and induced pluripotent stem cells Ex-vivo Expansion of Mesenchymal Stem Cells Stem Cells Reprogramming Stem Cell Banking Stem Cell and Regenerative Medicine
Umbilical Cord and Placenta	An Overview on Umbilical Cord Blood for Transplantation Cord Blood Banking An Overview on Amniotic and Placental Stem Cells The Placenta and the Fetus: Structure and Function Ethical Consideration of Umbilical Cord Blood Banking
Basics of Clinical Research	Fundamentals of Clinical Trials Principle and Practice of Clinical Research Regulatory Affairs in Clinical Research Ethics in Clinical Research Analysis of Clinical Data and Biostatistics
Regenerative Medicine	An Overview on Organ Failure Mechanisms of Cell Regeneration Nuclear Reprogramming and Developmental Plasticity Directed Differentiation of Stem Cells Commercial and Clinical Applications of Regenerative Medicine
Basics of Stem Cell Transplantations	Overview on the Types of Stem Cell Transplants Stem Cells Transplantation Process and Post-Management Ex-Vivo Expanded Stem Cell Transplantations Bioethics and Biosafety of Stem Cell Transplantations Autophagy in Stem Cells
Cell Therapies	Principles of Cell Therapies Application of Cell Therapies in Diabetes Application of Cell Therapies in Cancer Application of Cell Therapies in Cardiovascular Diseases Application of Cell Therapies in Wound Healing and Burn Damages

Course	Topics
Orthopedics	Orthopedics and Sports Medicine Cellular Therapies in Orthopedics
Transplantation	Basics of Bone Marrow Transplantation Procedure Comparative Analysis of Stem Cells from Cord Blood, Amniotic Fluid, and Peripheral Blood
Dermatology	Anti-aging Therapies Cellular Therapies in Burn Injuries
Hair/Skin	Hair Regeneration Skin Regeneration Ex-Vivo and In Vivo
Neurology	Underlying Mechanisms of Stroke and Possible Cellular Therapies Cellular Therapies in Neurodegenerative Disease and in Cognitive Functions
Oncology	Stem Cells and Cancer Radiobiology in Cancer Treatment
Cardiology	An Overview on Preventive Cardiology Cardiovascular Diseases
Pulmonary	Obstructive Lung Diseases Cell Therapies for Lung Diseases
Nephrology	Overview on Renal Diseases Cell Therapies for Kidney Diseases

Techniques

Sl No	Techniques
1	Aseptic Techniques in Cell Culture
2	Isolation of Mononuclear Cells/Stem Cells from Peripheral Blood/Bone Marrow/Adipose
3	Characterization of Stem Cells using Flow Cytometry
4	Expansion of Mesenchymal Stem Cells
5	Isolation and Cryopreservation of Stem Cells from Cord Blood
6	3D Printing
7	Extraction of Platelet Rich Plasma (PRP) from Peripheral Blood
8	Preparation of Platelet Rich Fibrin Matrix (PRFM) from Blood
9	Platelet Rich Plasma (PRP) Therapy for Androgenic Alopecia
10	Platelet Rich Plasma (PRP) Therapy for Skin Wrinkle Treatment
11	Stem Cell Based Therapies for Osteoarthritis
12	Isolation and Expansion of Hair Follicular Stem Cells
13	Isolation and Expansion of Melanocytes
14	Guidelines for Good Clinical Practice

Clinical Practicals

Sl No	Clinical Practicals
1	Application of PRP in Osteoarthritis
2	Isolation of Mononuclear Cells from Bone Marrow
3	Use of PRP in Hair/Skin
4	Application of PRP in Periodontal Disease
5	Autologous Platelet-Rich Fibrin Matrix in Non-Healing Diabetic Foot Ulcer
6	Infusion of Activated NK/T Cell Therapy in Cancer Patients